8.00AM - 6.00PM EST | 5.00AM - 3.00PM PST

8:50 am Chair’s Opening Remarks

  • Nicholas Harmer Associate Professor in Biochemistry, University of Exeter

Enhancing Industrial Applications and Solving Scale-Up Issues with Biocatalysed Reactions – Transitioning Biocatalytic Approaches from Lab to Commercial Manufacturing

9:00 am Biocatalysis for Commodity and Speciality Chemistry

Synopsis

  • Overview of the state of the art on the most famous biocatalytic processes currently at the industrial scale.
  • Explore a focus on a particular industrial scale-up for producing huge quantities of a compound of interest.
  • Highlight current R&D development at a lab-scale.

9:30 am Relevance of process modeling to biocatalysis at pilot scale

Synopsis

  • Discuss how even an item as basic as enzyme kinetics is not always accurately known when scaling a process
  • Assess Pareto front modeling and evaluation, common in other areas of chemical processing, has not been very prevalent in biocatalytic processing
  • Evaluate how such modeling can drive decisions about options for process improvement, with examples

10:00 am Novel approaches to oligonucleotide synthesis using enzymes

Synopsis

  • Oligonucleotides are rapidly degraded in the body and this has driven the need for chemical innovation to modify and stabilise these structures
  • Enzymes are now seeing applications beyond small molecules, such as exploited in oligonucleotide synthesis, and biocatalytic approaches are alleviating the pressures on existing solid phase capacity and resulting in more convergent syntheses
  • This strong technology base can be applied to develop enzymatic methods for oligonucleotide synthesis resulting in improved yields and purity profiles compared to traditional methods
  • This presentation will show case how enzymes can be used in both single and double stranded oligonucleotide synthesis with unnatural and natural nucleotides as well as carrying structural modifications

10:20 am Morning Networking Break

10:50 am Chair’s Opening Remarks

11:00 am Modifying Reaction Conditions to Enhancing Reactions and Avoiding Enzyme Engineering

  • Ryan Phelan Senior Scientist - Biocatalysis, Process Chem & Catalysis Group, AbbVie

Recent Advancements to Enhance Mature Reaction Classes

11:30 am Microbial lysyl oxidases: New Biocatalytic Tools for Chemical Biology, Materials Science And Beyond

  • Paul Race Professor - Biological Chemistry, University of Bristol

Synopsis

  • Learn how Lysyl oxidases (LOXs) catalyze the oxidative deamination of lysyl and hydroxylysyl groups, generating reactive aldehydes that undergo intermolecular cross-linking reactions
  • Discover how these enzymes are attractive candidates for use across a range of application areas, though their exploitation has been limited by challenges associated with their production at scale
  • Outline progress in the identification and recombinant production of LOXs from microbial sources, and describe examples of their use as tools in chemical biology tools and materials science

12:00 pm Lunch and Networking Break

1:00 pm Nitroreductase: Current Successes and Challenges at Amgen

Synopsis

  • Discuss what the industry has learned through the application of Nitroreductase technology over the last 2 years over a series of projects
  • Analyze the challenges that have been faced, the successes in the chemistry, and projections on where this technology is going

1:30 pm Late-Stage Chemoenzymatic Modifications of an FDAApproved Antibiotic

Synopsis

  • Uncover how Indole prenyltransferase (IPT) enzymes catalyze the transfer of prenyl groups from a native prenyl pyrophosphate donor to an indole-derived acceptor
  • Explore how IPTs have interesting promiscuity in terms of donors and acceptors allowing them to catalyze late-stage modifications of compounds.
  • Learn how to use a chemoenzymatic method involving different pyrophosphate substrates and IPTs to synthesize derivatives of an FDA-approved antibiotic, and how this makes derivatives possess superior antimicrobial activity compared to the parent compound.

2:00 pm Using Catalyst-Control for Selective C-Cl and C-C Bond Forming Reactions

  • Mary Andorfer Postdoctoral Researcher, Massachusetts Institute of Technology

Synopsis

  • Using directed evolution to change the site-selectivity of halogenases
  • Substrate-activity profiling of natural and engineered halogenases
  • Glycyl radical enzymes (GREs): Potentially powerful anaerobic, C-C bond-forming biocatalysts
  • How has nature overcome the extreme oxygen-sensitivity of GREs?

2:30 pm Exploration of Microbial Diversity of Ene-Reductases for Precious Metals Replacement

2:50 pm Afternoon Break

Utilising Enzyme Engineering to Optimise Novel Biotransformations

3:10 pm Biocatalytic Toolbox for Site-Selective Insulin Modification

  • Chihui An Associate Principal Scientist, Merck & Co

Synopsis

  • Outline the efforts on developing a general enzymatic method to chemoselectively functionalize insulin to enable the discovery and commercial manufacturing of novel insulin therapeutics
  • Explore how to use enzyme evolution to evolve from a single enzyme parent, a “toolbox” of highly active and selective amidation enzymes that enable chemists to construct insulin analogs without laborious chromatographic purification
  • Evaluate how the enzymes from the toolbox were applied to large scale synthesis of a preclinical candidate to demonstrate application for commercial manufacturing

3:40 pm Deep Dive into Machine Learning Models For Directed- Evolution Of Enzymes

Synopsis

  • Discuss the increased interest in using machine learning to assist in enzyme redesign in the pharmaceutical industry
  • Benchmark the performance of prediction models built using an array of machine learning methods and protein descriptor types (sequence and structure-based) against a variety of datasets
  • Evaluate the results suggest that Convolution Neural Network models built with amino acid property descriptors could be the most widely applicable technique for enzyme reengineering

4:10 pm Chair’s Closing Remarks